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Anaerobic Biooxidation of Fe(II) by Dechlorosoma suillum J.G. Lack, S.K. Chaudhuri, R. Chakraborty, L.A. Achenbach, J.D. Coates
 

Summary: Anaerobic Biooxidation of Fe(II) by Dechlorosoma suillum
J.G. Lack, S.K. Chaudhuri, R. Chakraborty, L.A. Achenbach, J.D. Coates
Department of Microbiology, Southern Illinois University, Carbondale, IL 62901, USA
Received: 26 September 2001; Accepted: 10 December 2001; Online publication: 15 April 2002
A B S T R A C T
Anaerobic microbial oxidation of Fe(II) was only recently discovered and very little is known
about this metabolism. We recently demonstrated that several dissimilatory perchlorate-re-
ducing bacteria could utilize Fe(II) as an electron donor under anaerobic conditions. Here we
report on a more in-depth analysis of Fe(II) oxidation by one of these organisms, Dechlorosoma
suillum. Similarly to most known nitrate-dependent Fe(II) oxidizers, D. suillum did not grow
heterotrophically or lithoautotrophically by anaerobic Fe(II) oxidation. In the absence of a
suitable organic carbon source, cells rapidly lysed even though nitrate-dependent Fe(II) oxi-
dation was still occurring. The coupling of Fe(II) oxidation to a particular electron acceptor was
dependent on the growth conditions of cells of D. suillum. As such, anaerobically grown cultures
of D. suillum did not mediate Fe(II) oxidation with oxygen as the electron acceptor, while
conversely, aerobically grown cultures did not mediate Fe(II) oxidation with nitrate as the
electron acceptor. Anaerobic washed cell suspensions of D. suillum rapidly produced an orange/
brown precipitate which X-ray diffraction analysis identiŽed as amorphous ferric oxyhydroxide
or ferrihydrite. This is similar to all other identiŽed nitrate-dependent Fe(II) oxidizers but is in
contrast to what is observed for growth cultures of D. suillum, which produced a mixed-valence

  

Source: Achenbach, Laurie A. - Department of Microbiology, Southern Illinois University

 

Collections: Environmental Sciences and Ecology; Environmental Management and Restoration Technologies; Biology and Medicine