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could reduce the required DNA amount, to-gether with use of the "breadth-first" search
 

Summary:  could reduce the required DNA amount, to-
gether with use of the "breadth-first" search
proposed for large SAT problems (6, 24),
where our algorithm must be repeated by the
number of the clauses, although several claus-
es can be processed simultaneously. Upon
tackling large instances, PCR may introduce
serious errors into computation because all
sequences may not be amplified with the same
efficiency (7). However, a variation in se-
quence was still retained after our computa-
tion including as many as 70 PCR cycles (25).
Further study is necessary for unraveling the
nature of the possible bias during PCR.
Another drawback with our method is the
incompleteness of our understanding of the na-
ture of hairpin molecules. The limit to the avail-
able length of hairpin remains to be determined;
the available data suggest that an intact double
helix of 30 base pairs is stable enough for clos-

  

Source: Ajayan, Pulickel M. - Department of Mechanical Engineering and Materials Science, Rice University

 

Collections: Materials Science