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Disruption of Toxoplasma gondii Parasitophorous Vacuoles by the Mouse

Summary: Disruption of Toxoplasma gondii
Parasitophorous Vacuoles by the Mouse
p47-Resistance GTPases
Sascha Martens1
, Iana Parvanova1
, Jens Zerrahn2
, Gareth Griffiths3
, Gudrun Schell4
, Gaby Reichmann4
Jonathan C. Howard1*
1 Institute for Genetics, University of Cologne, Cologne, Germany, 2 Max Planck Institute for Infection Biology, Berlin, Germany, 3 European Molecular Biology Laboratory,
Heidelberg, Germany, 4 Institute for Medical Microbiology, Heinrich Heine University Duesseldorf, Duesseldorf, Germany
The p47 GTPases are essential for interferon-c-induced cell-autonomous immunity against the protozoan parasite,
Toxoplasma gondii, in mice, but the mechanism of resistance is poorly understood. We show that the p47 GTPases,
including IIGP1, accumulate at vacuoles containing T. gondii. The accumulation is GTP-dependent and requires live
parasites. Vacuolar IIGP1 accumulations undergo a maturation-like process accompanied by vesiculation of the
parasitophorous vacuole membrane. This culminates in disruption of the parasitophorous vacuole and finally of the
parasite itself. Over-expression of IIGP1 leads to accelerated vacuolar disruption whereas a dominant negative form of
IIGP1 interferes with interferon-c-mediated killing of intracellular parasites. Targeted deletion of the IIGP1 gene results


Source: Arnold, Jonathan - Nanoscale Science and Engineering Center & Department of Genetics, University of Georgia


Collections: Biotechnology