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Effects of CO2 Concentration and Inoculum Stage on Chlorella sorokiniana

Summary: Effects of CO2 Concentration and Inoculum Stage on
Chlorella sorokiniana
Erico Mattos
Crop and Soil Sciences Seminar
Wednesday February 16, 2011 at 3:35 PM
Room 2401 Miller Plant sciences Building
Recently, there has been renewed interest in microalgal biofixation of CO2 as a viable
CO2 sequestration technology (Ono and Cuello 2006) in which CO2 emitted by an industrial
process is used as a source of inorganic carbon to stimulate microalgal growth. This process is
being investigated by scientists funded by the U.S. Department of Energy (Huesemann et al.,
2003). Microalgae are not a well-studied group from a biotechnological point of view. Of the
tens of thousands of microalgal species believed to exist, only a few thousand strains are kept in
collections around the world, and only a few have been cultivated in industrial quantities
(Olaizola 2003). We initiated a project with the overall objective of identifying microalgae
species tolerant to high CO2 concentrations. As a first step, we grew Chlorella sorokiniana for
30 days to identify the best time to collect inoculums from three growth phases: lag, exponential,
and stationary. In a following experiment, two variables affecting the overall biomass production
were investigated, including CO2 concentration (0.03%, 5% or 10% CO2 by volume) and
inoculum phase (lag, exponential and stationary). Results showed that the 5% CO2 concentration
with inoculum from the lag phase yielded the greatest biomass production. Future work will use


Source: Arnold, Jonathan - Nanoscale Science and Engineering Center & Department of Genetics, University of Georgia


Collections: Biotechnology