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Efficient screening of fungal cellobiohydrolase class I enzymes for thermostabilizing sequence blocks by
 

Summary: Efficient screening of fungal cellobiohydrolase class I
enzymes for thermostabilizing sequence blocks by
SCHEMA structure-guided recombination
Pete Heinzelman, Russell Komor, Arvind Kanaan,
Philip Romero, Xinlin Yu, Shannon Mohler,
Christopher Snow and Frances Arnold1
Division of Chemistry and Chemical Engineering, California Institute of
Technology 210-41, Pasadena, CA 91125, USA
1
To whom correspondence should be addressed.
E-mail: frances@cheme.caltech.edu
Received August 18, 2010; revised August 18, 2010;
accepted August 24, 2010
Edited by Stephen Withers
We describe an efficient SCHEMA recombination-based
approach for screening homologous enzymes to identify
stabilizing amino acid sequence blocks. This approach
has been used to generate active, thermostable cellobiohy-
drolase class I (CBH I) enzymes from the 390 625 poss-
ible chimeras that can be made by swapping eight blocks

  

Source: Arnold, Frances H. - Division of Chemistry and Chemical Engineering, California Institute of Technology

 

Collections: Chemistry; Biology and Medicine