Summary: Current Biology, Vol. 12, 2035­2041, December 10, 2002, 2002 Elsevier Science Ltd. All rights reserved. PII S0960-9822(02)01333-7
Rapid Coevolution of the Nematode
Sex-Determining Genes fem-3 and tra-2
notable for the hypervariability of their FEM-3 binding
domains. Similar sequence divergence likely thwarted
previous efforts to identify fem-3 homologs, leading us
Eric S. Haag,1,2,3,4
Shanping Wang,2
and Judith Kimble,1,2
1
Department of Biochemistry and
to use a genomics-based synteny approach [13]. At-2
Howard Hughes Medical Institute
tempts to identify fem-3 homologs by using the C. ele-University of Wisconsin
gans ion channel gene twk-19 were thwarted by a break-433 Babcock Drive
age in synteny relative to C. elegans in both C. briggsaeMadison, Wisconsin 53706
and C. remanei (Figure 2A). We next used the predicted
C. elegans gene C01F6.1, which lies 7 kb from Ce-fem-3
and encodes a copine family protein [14]. Low-strin-Summary
gency hybridization with a C01F6.1 fragment produced

Source: Azevedo, Ricardo - Department of Biology and Biochemistry, University of Houston
Kimble, Judith - Department of Biochemistry, University of Wisconsin at Madison

Collections: Biology and Medicine; Environmental Sciences and Ecology