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Evaluation of the RNA Determinants for Bacterial and Yeast RNase III Binding and Cleavage*S
 

Summary: Evaluation of the RNA Determinants for Bacterial and Yeast
RNase III Binding and Cleavage*S
Received for publication, August 22, 2003, and in revised form, October 21, 2003
Published, JBC Papers in Press, October 27, 2003, DOI 10.1074/jbc.M309324200
Bruno Lamontagne and Sherif Abou Elela§
From the Groupe ARN/RNA Group, De´partement de Microbiologie et d'Infectiologie, Faculte´ de Me´decine,
Universite´ de Sherbrooke, Sherbrooke, Que´bec J1H 5N4, Canada
Bacterial double-stranded RNA-specific RNase III rec-
ognizes the A-form of an RNA helix with little sequence
specificity. In contrast, baker yeast RNase III (Rnt1p)
selectively recognizes NGNN tetraloops even when they
are attached to a B-form DNA helix. To comprehend the
general mechanism of RNase III substrate recognition,
we mapped the Rnt1p binding signal and directly com-
pared its substrate specificity to that of both Esche-
richia coli RNase III and fission yeast RNase III (PacI).
Rnt1p bound but did not cleave long RNA duplexes with-
out NGNN tetraloops, whereas RNase III indiscrimi-
nately cleaved all RNA duplexes. PacI cleaved RNA du-
plexes with some preferences for NGNN-capped RNA

  

Source: Abou Elela, Sherif - Département de Microbiologie et d'Infectiologie, Université de Sherbrooke

 

Collections: Biology and Medicine