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Identification and Genome-Wide Prediction of DNA Binding Specificities for the ApiAP2 Family of Regulators

Summary: Identification and Genome-Wide Prediction of DNA
Binding Specificities for the ApiAP2 Family of Regulators
from the Malaria Parasite
Tracey L. Campbell1
, Erandi K. De Silva1
, Kellen L. Olszewski1
, Olivier Elemento2
, Manuel Llina´s1
1 Department of Molecular Biology & Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, United States of America, 2 Institute for
Computational Medicine, Weill Cornell Medical College, New York, New York, United States of America
The molecular mechanisms underlying transcriptional regulation in apicomplexan parasites remain poorly understood.
Recently, the Apicomplexan AP2 (ApiAP2) family of DNA binding proteins was identified as a major class of transcriptional
regulators that are found across all Apicomplexa. To gain insight into the regulatory role of these proteins in the malaria
parasite, we have comprehensively surveyed the DNA-binding specificities of all 27 members of the ApiAP2 protein family
from Plasmodium falciparum revealing unique binding preferences for the majority of these DNA binding proteins. In
addition to high affinity primary motif interactions, we also observe interactions with secondary motifs. The ability of a
number of ApiAP2 proteins to bind multiple, distinct motifs significantly increases the potential complexity of the
transcriptional regulatory networks governed by the ApiAP2 family. Using these newly identified sequence motifs, we infer


Source: Arnold, Jonathan - Nanoscale Science and Engineering Center & Department of Genetics, University of Georgia


Collections: Biotechnology