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Summary: Kinetics of 1,4-Dioxane
Biodegradation by
Monooxygenase-Expressing Bacteria
S H A I L Y M A H E N D R A * A N D
L I S A A L V A R E Z - C O H E N
Department of Civil and Environmental Engineering,
726 Davis Hall, University of California,
Berkeley, California 94720-1710
1,4-Dioxane is a probable human carcinogen, and an
important emerging water contaminant. In this study, the
biodegradation of dioxane by 20 bacterial isolates was
evaluated, and 13 were found to be capable of transforming
dioxane. Dioxane served as a growth substrate for
Pseudonocardia dioxanivorans CB1190 and Pseudonocardia
benzenivorans B5, with yields of 0.09 g protein g dioxane-1
and 0.03 g protein g dioxane-1, respectively. Cometabolic
transformation of dioxane was observed for monooxygenase-
expressing strains that were induced with methane,
propane, tetrahydrofuran, or toluene including Methylosinus
trichosporium OB3b, Mycobacterium vaccae JOB5,
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