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Vol. 153, No. 1JOURNAL OF BACTERIOLOGY, Jan. 1983, p. 45-56 0021-9193/83/010045-12$02.00/0
 

Summary: Vol. 153, No. 1JOURNAL OF BACTERIOLOGY, Jan. 1983, p. 45-56
0021-9193/83/010045-12$02.00/0
Copyright 1983, American Society for Microbiology
Nitrogen Fixation by Klebsiella pneumoniae Is Inhibited by
Certain Multicopy Hybrid nif Plasmids
GERARD E. RIEDEL,12t* SUSAN E. BROWN,1 AND FREDERICK M. AUSUBEL'
Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 021381
and Division ofPlant Industry, Commonwealth Scientific and Industrial Research Organization, Canberra
City, A.C.T. 2601, Australia2
Received 20 July 1982/Accepted 16 September 1982
In our studies of nifgene regulation, we have observed that certain hybrid nif
plasmids drastically inhibit the expression of the chromosomal nif genes of
Klebsiella pneumonia. Wild-type (Nif) K. pneumoniae strains that acquire
certain hybrid nifplasmids also acquire the Nif- phenotype; these strains lose 90
to 99% ofall detectable nitrogen fixation activity and grow poorly (or not at all) on
solid media with N2 as the sole nitrogen source. We describe experiments which
defined this inhibition of the Nif+ phenotype by hybrid nifplasmids and identify
and characterize four nifDNA regions associated with this inhibition. We show
that plasmids carrying these nifregions could recombine with, but not comple-
ment, nifchromosomal mutations. Our results suggest that inhibition of the Nif+

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine