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Bacteria Media Media Sterilization
 

Summary: 1
Bacteria Media
Media Sterilization
Use the following exposure times for liquid media and remove immediately at end of liquid cycle.
Volume Vessel Exposure Time
100 ml Glass Bottle/Flask 25 minutes
500 ml Glass Bottle/Flask 35 minutes
1 L Plastic Fernbach Flask 45 minutes
For Plates: Add 16 g Bacto-agar per liter. For large volumes, add stir bar.
LB (Luria-Bertani): 1 L
5 g Yeast Extract
10 g Tryptone
5 g NaCl
2.5 ml 1M KOH
LBPD:
Adjust volume to 950 ml. Autoclave and cool (to 50C for plates).
Add 10 ml 1M KH2PO4 + 40 ml 1 M K2HPO4 (pH ~7.5). Add 5 ml 20% dextrose.
YETM: 1L
5 g YE
20 g Tryptone

  

Source: Aris, John P. - Department of Anatomy and Cell Biology, University of Florida

 

Collections: Biology and Medicine