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UCSD Transgenic Mouse and Gene Targeting Core
 

Summary: UCSD Transgenic Mouse and
Gene Targeting Core
9500 Gilman Drive, MC 0687
La Jolla, CA 92093-0687
(858) 534-3178
Current as of 2/03 1
Genomic DNA Preparation from Mouse Tissue
for PCR Analysis Only
(Phenol-free DNA Preparation)
Note: This method works well for small embryos (6.5, 7.5 or 8.5 dpc). It is also good for
2mm chunks of tail. This method will not work with bigger embryos (9.5 or older)
because they yield too much DNA (or protein). Best PCR results when you take just part
of the embryo (e.g. yolk sac or head).
Reagents
NaOH Solution: 25mM NaOH + 2 mM EDTA
Tris Solution: 40 mM Tris-HCl
Method
1. Use only clean Eppendorf tubes. Set up tubes in rack and have lids closed. Label
tops of tubes.
2. Cut off toe or tip of tail and place into Eppendorf tube and cap. You can freeze

  

Source: Abagyan, Ruben - School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego

 

Collections: Biology and Medicine