Summary: White-core endosperm floury endosperm-4 in rice is
generated by knockout mutations in the C4-type pyruvate
orthophosphate dikinase gene (OsPPDKB)
, Sunhee Park1
, Makoto Matsuoka2
and Gynheung An1,*
Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang University of Science and
Technology, Pohang 790-784, Korea, and
Bioscience Center, Nagoya University, Aichi 464-01, Japan
Received 13 February 2005; revised 17 March 2005; accepted 23 March 2005.
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We have isolated a floury endosperm-4 (flo4) rice mutant with a floury-white endosperm but a normal outer
portion. Scanning electron microscopic analysis revealed that this abnormal endosperm consisted of loosely
packed starch granules. The mutant phenotype was generated by T-DNA insertion into the fifth intron of the
OsPPDKB gene encoding pyruvate orthophosphate dikinase (PPDK). Plants containing flo4-1 produced no