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Summary: Determination of the Motif Responsible for Interaction
between the Rice APETALA1/AGAMOUS-LIKE9 Family
Proteins Using a Yeast Two-Hybrid System1
Yong-Hwan Moon, Hong-Gyu Kang, Ji-Young Jung, Jong-Seong Jeon, Soon-Kee Sung, and Gynheung An*
Department of Life Science, Pohang University of Science and Technology, Pohang 790784, Republic of Korea
A MADS family gene, OsMADS6, was isolated from a rice (Oryza
sativa L.) young flower cDNA library using OsAMDS1 as a probe.
With this clone, various MADS box genes that encode for protein-
to-protein interaction partners of the OsMADS6 protein were iso-
lated by the yeast two-hybrid screening method. On the basis of
sequence homology, OsMADS6 and the selected partners can be
classified in the APETALA1/AGAMOUS-LIKE9 (AP1/AGL9) family.
One of the interaction partners, OsMADS14, was selected for fur-
ther study. Both genes began expression at early stages of flower
development, and their expression was extended into the later
stages. In mature flowers the OsMADS6 transcript was detectable in
lodicules and also weakly in sterile lemmas and carpels, whereas the
OsMADS14 transcript was detectable in sterile lemmas, paleas/
lemmas, stamens, and carpels. Using the yeast two-hybrid system,
we demonstrated that the region containing of the 109th to 137th
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