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NATURE BIOTECHNOLOGY VOL 17 JULY 1999 http://biotech.nature.com 683 Many of the problems currently studied in molecular biology and
 

Summary: NATURE BIOTECHNOLOGY VOL 17 JULY 1999 http://biotech.nature.com 683
Many of the problems currently studied in molecular biology and
biochemistry are governed by protein­protein interactions.
Important examples are the identification and functional charac-
terization of novel gene products, the dissection of proteins into
structural or functional motifs, and the study of the physical basis
of protein­protein complementarity, whether in naturally occur-
ring proteins or in designed products. The ability to address these
problems has been transformed by the development of peptide-
and protein-library screening techniques such as the yeast two-
hybrid strategy1,2 and phage display3, in which a library of proteins
is screened for interaction with a "bait" protein. However, as the
study of interacting partners is a two-dimensional problem influ-
enced by variations in either partner, it would be advantageous to
screen a library of proteins not against a single bait protein, but
against a second library of proteins. To date, no large-scale library-
versus-library selection of protein­protein interactions has been
reported.
We present a strategy for library-versus-library screening in
intact cells based on the folding of murine enzyme dihydrofolate

  

Source: Arndt, Katja - Institut für Biologie III, Albert-Ludwigs-Universität Freiburg

 

Collections: Biotechnology; Biology and Medicine