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MOLECULAR AND CELLULAR BIOLOGY, July 2004, p. 63626378 Vol. 24, No. 14 0270-7306/04/$08.00 0 DOI: 10.1128/MCB.24.14.63626378.2004
 

Summary: MOLECULAR AND CELLULAR BIOLOGY, July 2004, p. 63626378 Vol. 24, No. 14
0270-7306/04/$08.00 0 DOI: 10.1128/MCB.24.14.63626378.2004
Copyright 2004, American Society for Microbiology. All Rights Reserved.
The NEF4 Complex Regulates Rad4 Levels and Utilizes
Snf2/Swi2-Related ATPase Activity for Nucleotide
Excision Repair
Kerrington L. Ramsey, Joshua J. Smith, Arindam Dasgupta, Nazif Maqani,
Patrick Grant, and David T. Auble*
Department of Biochemistry and Molecular Genetics, University of Virginia Health System,
Charlottesville, Virginia 22908-0733
Received 23 March 2004/Accepted 20 April 2004
Nucleotide excision repair factor 4 (NEF4) is required for repair of nontranscribed DNA in Saccharomyces
cerevisiae. Rad7 and the Snf2/Swi2-related ATPase Rad16 are NEF4 subunits. We report previously unrecog-
nized similarity between Rad7 and F-box proteins. Rad16 contains a RING domain embedded within its
ATPase domain, and the presence of these motifs in NEF4 suggested that NEF4 functions as both an ATPase
and an E3 ubiquitin ligase. Mutational analysis provides strong support for this model. The Rad16 ATPase is
important for NEF4 function in vivo, and genetic analysis uncovered new interactions between NEF4 and
Rad23, a repair factor that links repair to proteasome function. Elc1 is the yeast homologue of a mammalian
E3 subunit, and it is a novel component of NEF4. Moreover, the E2s Ubc9 and Ubc13 were linked to the NEF4
repair pathway by genetic criteria. Mutations in NEF4 or Ubc13 result in elevated levels of the DNA damage

  

Source: Auble, David - Department of Biochemistry and Molecular Genetics, University of Virginia

 

Collections: Biology and Medicine