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APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Sept. 2004, p. 56515658 Vol. 70, No. 9 0099-2240/04/$08.00 0 DOI: 10.1128/AEM.70.9.56515658.2004
 

Summary: APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Sept. 2004, p. 56515658 Vol. 70, No. 9
0099-2240/04/$08.00 0 DOI: 10.1128/AEM.70.9.56515658.2004
Copyright 2004, American Society for Microbiology. All Rights Reserved.
Metabolic Primers for Detection of (Per)chlorate-Reducing Bacteria in
the Environment and Phylogenetic Analysis of cld Gene Sequences
Kelly S. Bender,1
Melissa R. Rice,1
William H. Fugate,1
John D. Coates,2
and Laurie A. Achenbach1
*
Department of Microbiology, Southern Illinois University, Carbondale, Illinois,1
and Department of Plant and Microbial Biology,
University of California Berkeley, Berkeley, California2
Received 26 April 2004/Accepted 22 May 2004
Natural attenuation of the environmental contaminant perchlorate is a cost-effective alternative to current
removal methods. The success of natural perchlorate remediation is dependent on the presence and activity of
dissimilatory (per)chlorate-reducing bacteria (DPRB) within a target site. To detect DPRB in the environment,
two degenerate primer sets targeting the chlorite dismutase (cld) gene were developed and optimized. A nested
PCR approach was used in conjunction with these primer sets to increase the sensitivity of the molecular

  

Source: Achenbach, Laurie A. - Department of Microbiology, Southern Illinois University

 

Collections: Environmental Sciences and Ecology; Environmental Management and Restoration Technologies; Biology and Medicine