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APPLIED AND ENVIRONMENTAL MICROBIOLOGY, June 2010, p. 40634075 Vol. 76, No. 12 0099-2240/10/$12.00 doi:10.1128/AEM.02928-09
 

Summary: APPLIED AND ENVIRONMENTAL MICROBIOLOGY, June 2010, p. 40634075 Vol. 76, No. 12
0099-2240/10/$12.00 doi:10.1128/AEM.02928-09
Copyright 2010, American Society for Microbiology. All Rights Reserved.
Diverse Bacteria Inhabit Living Hyphae of Phylogenetically
Diverse Fungal Endophytes
Michele T. Hoffman and A. Elizabeth Arnold*
Division of Plant Pathology and Microbiology, School of Plant Sciences, 1140 E. South Campus Drive,
University of Arizona, Tucson, Arizona 85721
Received 3 December 2009/Accepted 20 April 2010
Both the establishment and outcomes of plant-fungus symbioses can be influenced by abiotic factors, the
interplay of fungal and plant genotypes, and additional microbes associated with fungal mycelia. Recently
bacterial endosymbionts were documented in soilborne Glomeromycota and Mucoromycotina and in at least
one species each of mycorrhizal Basidiomycota and Ascomycota. Here we show for the first time that phylo-
genetically diverse endohyphal bacteria occur in living hyphae of diverse foliar endophytes, including repre-
sentatives of four classes of Ascomycota. We examined 414 isolates of endophytic fungi, isolated from photo-
synthetic tissues of six species of cupressaceous trees in five biogeographic provinces, for endohyphal bacteria
using microscopy and molecular techniques. Viable bacteria were observed within living hyphae of endophytic
Pezizomycetes, Dothideomycetes, Eurotiomycetes, and Sordariomycetes from all tree species and biotic regions
surveyed. A focus on 29 fungus/bacterium associations revealed that bacterial and fungal phylogenies were
incongruent with each other and with taxonomic relationships of host plants. Overall, eight families and 15

  

Source: Arnold, A. Elizabeth - School of Plant Sciences, University of Arizona

 

Collections: Biology and Medicine