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Fish & Shellfish Immunology (2002) 13, 141158 doi:10.1006/fsim.2001.0389
 

Summary: Fish & Shellfish Immunology (2002) 13, 141158
doi:10.1006/fsim.2001.0389
Available online at http://www.idealibrary.com on
Flow cytometric comparison of haemocytes from three
species of bivalve molluscs
B. ALLAM, K. A. ASHTON-ALCOX AND S. E. FORD*
Institute of Marine and Coastal Sciences, Rutgers University, Haskin
Shellfish Research Laboratory, 6959 Miller Avenue, Port Norris,
NJ 08349-3617, U.S.A.
(Received 27 August 2001, accepted 29 October 2001,
published electronically 2001)
Haemocyte subpopulations from three bivalve species (the clams Ruditapes
philippinarum and Mercenaria mercenaria and the oyster, Crassostrea virgi-
nica) were characterised using light-scatter flow cytometry and a standard set
of methods. Two parameter (forward and side scatter) plots for the three
species were very similar and resembled plots for mammalian white blood
cells. Two haemocyte groups (granulocytes and agranulocytes) were found in
both the haemolymph and the extrapallial fluid of the clams while those two
groups and an additional third group were found in the haemolymph of the
oyster. All subpopulations were sorted on to glass slides, identified, photo-

  

Source: Allam, Bassem - School of Marine and Atmospheric Sciences, SUNY at Stony Brook

 

Collections: Biology and Medicine; Environmental Sciences and Ecology