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PRECISE LOCALIZATION OF FLUORESCENT PROBES WITHOUT NUMERICAL FITTING Sean B. Andersson
 

Summary: PRECISE LOCALIZATION OF FLUORESCENT PROBES WITHOUT NUMERICAL FITTING
Sean B. Andersson
Aerospace and Mechanical Eng., Boston University, Boston, MA 02215
ABSTRACT
A new algorithm for determining the location of a fluorescent
probe in the plane is described. Inspired by the Bancroft al-
gorithm for localization in the global positioning system, the
technique calculates the position of the probe without the use
of a numerical fitting procedure. Numerical simulations are
presented which indicate that the precision of the localization
is similar to that obtained using the standard method of fit-
ting a Gaussian profile to the measured fluorescence intensity.
Moreover, the computational time is two orders of magnitude
shorter than when performing such a fit.
Keywords: biomedical microscopy, biomedical imaging
1. INTRODUCTION
The resolution of a visible light microscope is limited to ap-
proximately half the wavelength of the light [1]. An object
smaller than this appears as a diffraction-limited spot. While
the structural details of such an object cannot be distinguished,

  

Source: Andersson, Sean B. - Department of Aerospace and Mechanical Engineering, Boston University

 

Collections: Engineering