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Characterization of the Physicochemical Parameters of Dense Core Atrial Gland and Lucent
 

Summary: Characterization of the Physicochemical
Parameters of Dense Core Atrial Gland and Lucent
Red Hemiduct Vesicles in Aplysia californica
Luisa Ciobanu,, Stanislav S. Rubakhin,, Jeffrey N. Stuart,, Robert R. Fuller,,,|
Andrew G. Webb,*,, and Jonathan V. Sweedler*,,
Department of Electrical and Computer Engineering, University of Illinois at UrbanasChampaign, 1406 West Green Street,
Urbana, Illinois 61801, Beckman Institute, University of Illinois at UrbanasChampaign, 405 North Mathews Avenue,
Urbana, Illinois 61801, and Department of Chemistry, University of Illinois at UrbanasChampaign, 600 South Mathews
Avenue, Urbana, Illinois 61801
Characterizing femtoliter-volume cellular organelles re-
quires innovative analytical techniques such as mass
spectrometry, separations, and NMR. The capabilities of
all three are demonstrated for characterizing the physi-
cochemical properties of the electron-dense core atrial
gland vesicles from Aplysia californica and comparing
them with the same properties of the electron lucent red
hemiduct vesicles. Single-vesicle mass spectrometric
measurements show that the atrial gland vesicles contain
an abundance of peptides while the red hemiduct vesicles
contain no detectable peptide signals. Capillary electro-

  

Source: Andrews, Anne M. - Huck Institutes of the Life Sciences, Pennsylvania State University

 

Collections: Biology and Medicine