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Supplementary Methods Neural recordings

Summary: Supplementary Methods
Neural recordings
Neural recordings were made using Pt/Ir electrodes in multiple-electrode microdrives (3-
or 5-channel, Thomas Recordings, Germany). Neural activity was referenced to the
guide tube in each chamber which was in contact with the cerebral spinal fluid. During
each session neural activity from each electrode was passed through a headstage (x20,
Thomas Recordings, Germany), filtered (1 Hz 10 kHz; custom), amplified (x500-1000;
TDT Electronics, Gainesville, FL), digitized (20 kHz; National Instruments, TX) and
continuously recorded to disk for further analysis (custom C and Matlab code).
At a subset of recording sites in the frontal chamber of each monkey,
microstimulation through the recording electrode evoked movements of the hand, arm
and occasionally leg with a threshold > 40 uA (330 Hz 400 us monopolar pulse width)
consistent with published reports for PMd1
. With a low amplitude <80 uA pulse,
saccades were not evoked at any recording site, consistent with published reports for the
caudal subdivision of PMd 2
. Single cell recordings from both areas in both animals
contained visual-, delay- and reach-related responses.
It is possible that the phase of the LFP signal is seriously distorted due to electrode
polarization and signal conditioning by the recording hardware 3,4


Source: Andersen, Richard - Division of Biology, California Institute of Technology


Collections: Biology and Medicine