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A Novel Plant Cysteine Protease Has a Dual Function as a Regulator of 1-Aminocyclopropane-1-Carboxylic Acid
 

Summary: A Novel Plant Cysteine Protease Has a Dual Function as
a Regulator of 1-Aminocyclopropane-1-Carboxylic Acid
Synthase Gene Expression W
Noa Matarasso, Silvia Schuster, and Adi Avni1
Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel
The hormone ethylene influences plant growth, development, and some defense responses. The fungal elicitor Ethylene-
Inducing Xylanase (EIX) elicits ethylene biosynthesis in tomato (Lycopersicon esculentum) and tobacco (Nicotiana tabacum)
leaves by induction of 1-aminocyclopropane-1-caboxylic acid synthase (Acs) gene expression. A minimal promoter element
in the LeAcs2 gene required for EIX responsiveness was defined by deletion analysis in transgenic tomato plants. The
sequence between 715 and 675 of the tomato Acs2 gene was found to be essential for induction by EIX. A Cys protease
(LeCp) was isolated that specifically binds to this cis element in vitro. Ectopic expression of LeCp in tomato leaves induced
the expression of Acs2. Moreover, chromatin immunoprecipitation showed that LeCp binds in vivo to the Acs promoter. We
propose a mechanism for the dual function of the LeCp protein. The protease acts enzymatically in the cytoplasm. Then,
upon signaling, a small ubiquitin-related modifier protein binds to it, enabling entrance into the nucleus, where it acts as
a transcription factor. Thus, LeCp can be considered a dual-function protein, having enzymatic activity and, upon elicitor
signaling, exhibiting transcriptional factor activity that induces LeAcs2 expression.
INTRODUCTION
Ethylene as a plant hormone influences many aspects of plant
growth and development (Mattoo and Suttle, 1991; Abeles et al.,
1992; Bleecker and Kende, 2000; Mattoo and Handa, 2003) as

  

Source: Avni, Adi - Department of Plant Sciences, Tel Aviv University

 

Collections: Biology and Medicine