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The Center for Control, Dynamical Systems, and Computation University of California at Santa Barbara
 

Summary: The Center for Control, Dynamical Systems, and Computation
University of California at Santa Barbara
Spring 2010 Seminar Series
Presents
Measuring and Controlling Single Molecule Dynamics with
a Nanopore
William Dunbar
UC Santa Cruz
Friday, June 4, 2010, 3:00 4:00pm WEBB 1100
Abstract:
The nanopore is an established tool for studying the kinetic properties of polynucleotide and polynucleotide-
protein molecules at the single molecule level, and continues to show great promise for inexpensive genomic
sequencing. Our experiments are the first to combine the nanopore with control logic that enables control over
the temporal availability of a single DNA molecule, trapped in the nanopore, to associating molecules in solution
above the nanopore. Using this approach, I will present our results in studying the kinetics of DNA hybridization
and of DNA-DNA polymerase assembly. The method is statistically rich: tens of thousands of molecular interac-
tions can be measured during a 1 hour experiment. From the measured distributions, I will describe our efforts
to uncover and model the kinetic states and state transition parameters (equilibrium and rate constants) for the
molecular complex being studied. Using tools from systems and control theory, future work will aim toward online
estimation and regulation of the state(s) of the molecule.

  

Source: Akhmedov, Azer - Department of Mathematics, University of California at Santa Barbara

 

Collections: Mathematics