Summary: Supporting Information
© Wiley-VCH 2007
69451 Weinheim, Germany
Engineered alkane-hydroxylating cytochrome P450BM3 exhibiting native-like catalytic
Rudi Fasan, Mike M. Chen, Nathan C. Crook, Frances H. Arnold*
Department of Chemistry and Chemical Engineering, California Institute of Technology, 91125 Pasadena (CA), U.S.A.
Plasmids and oligonucleotides. Plasmid pCWori
was used as cloning vector. Oligonucleotides were purchased from
Invitrogen (Carlsbad, CA). The sequence of the primers used in this study are reported below:
# Primer name Sequence
1 L52I_for 5'-CGC GCT ACA TAT CAA GTC AGC-3'
2 L52I_rev 5'-GCT GAC TTG ATA TGT AGC GCG-3'
3 M145A_for 5'-GTA TCG GAA GAC GCG ACA CGT TTA ACG-3'
4 M145A_rev 5'-GTA TCG GAA GAC GCG ACA CGT TTA ACG-3'
5 V340M_for 5'-GAA GAT ACG ATG CTT GGA GGA G-3'
6 V340M_rev 5'-CTC CTC CAA GCA TCG TAT CTT C-3'
7 I366V_for 5'-CGT GAT AAA ACA GTT TGG GGA GAC G-3'
8 I366V_rev 5'-CGT CTC CCC AAA CTG TTT TAT CAC G-3'