Home

About

Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network
FAQHELPSITE MAPCONTACT US


  Advanced Search  

 
Supporting Information Wiley-VCH 2007
 

Summary: Supporting Information
Wiley-VCH 2007
69451 Weinheim, Germany
Engineered alkane-hydroxylating cytochrome P450BM3 exhibiting native-like catalytic
properties
Rudi Fasan, Mike M. Chen, Nathan C. Crook, Frances H. Arnold*
Department of Chemistry and Chemical Engineering, California Institute of Technology, 91125 Pasadena (CA), U.S.A.
Experimental Section
Plasmids and oligonucleotides. Plasmid pCWori[1]
was used as cloning vector. Oligonucleotides were purchased from
Invitrogen (Carlsbad, CA). The sequence of the primers used in this study are reported below:
# Primer name Sequence
1 L52I_for 5'-CGC GCT ACA TAT CAA GTC AGC-3'
2 L52I_rev 5'-GCT GAC TTG ATA TGT AGC GCG-3'
3 M145A_for 5'-GTA TCG GAA GAC GCG ACA CGT TTA ACG-3'
4 M145A_rev 5'-GTA TCG GAA GAC GCG ACA CGT TTA ACG-3'
5 V340M_for 5'-GAA GAT ACG ATG CTT GGA GGA G-3'
6 V340M_rev 5'-CTC CTC CAA GCA TCG TAT CTT C-3'
7 I366V_for 5'-CGT GAT AAA ACA GTT TGG GGA GAC G-3'
8 I366V_rev 5'-CGT CTC CCC AAA CTG TTT TAT CAC G-3'

  

Source: Arnold, Frances H. - Division of Chemistry and Chemical Engineering, California Institute of Technology

 

Collections: Chemistry; Biology and Medicine