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DOI: 10.1002/cbic.201000565 Combinatorial Alanine Substitution Enables Rapid Optimization of
 

Summary: DOI: 10.1002/cbic.201000565
Combinatorial Alanine Substitution Enables Rapid Optimization of
Cytochrome P450BM3 for Selective Hydroxylation of Large Substrates
Jared C. Lewis,[d]
Simone M. Mantovani,[e]
Yu Fu,[b]
Christopher D. Snow,[a]
Russell S. Komor,[a]
Chi-Huey Wong,[b, c]
and Frances H. Arnold*[a]
Selective hydroxylation of CH bonds in organic compounds
provides an efficient means to access valuable drug metabo-
lites, natural product derivatives, and other fine chemicals.[1]
While chemical methods to accomplish this transformation
have improved, these generally require the presence of direct-
ing groups or electronic properties inherent to certain sub-
strate classes for the desired transformations to occur at all or
with useful regioselectivity.[2]
Enzymes are capable of avoiding
this limitation by employing potent H-abstraction mechanisms

  

Source: Arnold, Frances H. - Division of Chemistry and Chemical Engineering, California Institute of Technology

 

Collections: Chemistry; Biology and Medicine