Summary: APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Sept. 2006, p. 61616168 Vol. 72, No. 9
0099-2240/06/$08.00 0 doi:10.1128/AEM.01070-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Reductive Dehalogenase Gene Expression as a Biomarker
for Physiological Activity of Dehalococcoides spp.
Patrick K. H. Lee,1
David R. Johnson,1
Victor F. Holmes,1
and Lisa Alvarez-Cohen1,2
Department of Civil and Environmental Engineering, University of California, Berkeley, California 94720-1170,1
Earth Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd., Berkeley, California 947202
Received 9 May 2006/Accepted 29 June 2006
This study characterizes the transcriptional expression of the reductive dehalogenase (RDase)-encoding tceA
and vcrA genes and evaluates their applicability as potential biological markers of Dehalococcoides activity.
When Dehalococcoides ethenogenes 195 was provided with trichloroethene (TCE) as the electron acceptor, the
expression of the tceA gene increased by 90-fold relative to that in cells starved of chlorinated ethenes,
demonstrating that tceA gene expression is indicative of the active physiological state of this strain. In a