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Summary: Proc. Nati. Acad. Sci. USA
Vol. 75, No. 7, pp. 3158-3162, July 1978
Biochemistry
Cycloheximide resistance can be mediated through either
ribosomal subunit
[Tetrahymena thermophila/poly(U-directed poly(phenylalanine) synthesis/eukaryotic ribosomel
CLAUDIA A. SUTTON, MANUEL ARES, JR.*, AND RICHARD L. HALLBERGt
Section of Botany, Genetics and Development, Cornell University, Ithaca, New York 14853
Communicated by Donald D. Brown, April 28, 1978
ABSTRACT Two cycloheximide-resistant mutants of Te-
trahymena thermophila were analyzed to determine the site
of their cycloheximide resistance. The mutations in both strains
had been previously shown to be genetically dominant and lo-
cated at separate loci (denoted Chx-A and Chx-B). Strains
carrying these mutations were readily distinguished by the ex-
tent to which they were resistant to the drug. The homozygous
double mutant was more resistant than either single mutant.
Cell-free extracts of wild type and of the three mutant strains,
assayed for protein synthetic activity by both runoff of natural
mRNA and poly(U)dependent phenylalanine polymerization,
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