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Summary: NEST Scientific Report 2007-2009
En
GFP: protein nanodevices tailored for intracellular biosensing
79
O
wing to their genetically encoded fluorescence, green fluorescent proteins (GFPs)
have become an invaluable tool for the optical labeling and tracking of protein
molecules in living cells at high spatial and temporal resolution. One of the
ultimate challenges in microscopy is to devise GFP-based biosensors that combine the high
resolution imaging property of luminescent probes with the functional information afforded
by biochemical analysis. Here we present the development of new GFP-based biosensors
for high-resolution intracellular pH and chloride sensing. The spectral properties of the
En
GFP class (F64L/T203Y) were exploited for the design of a multiplex pH and chloride
sensor, which overcame the limitations of existing methods to sense intracellular chloride
concentration and pH.
E1
GFP as targeted biosensor of intracellular
pH
The chromophore of En
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