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So you want to do an immunohistochemistry assay? If the antibody has not been tested on tissues before, it will take quite a b it of effort to
 

Summary: So you want to do an immunohistochemistry assay?
If the antibody has not been tested on tissues before, it will take quite a b it of effort to
determine if the antibody is specific--- the assays will need to be optimized.
There should be a plan
--for negative and positive control reagents,
--tissue negative and positive controls,
--secondary reagents,
--blocking reagents
--are overnight incubations needed?
--will a fluorescence OR enzyme detection system be used?
--will amplifications such as antigen retrieval
--or will catalyzed signal amplification be needed?
etc. etc.
You may find some hints on the powerpoint presentations on the histology core website:
http://cancer.ucsd.edu/histology/
Other Questions:
1.Are you going to immunostain frozen sections or paraffin sections?
2. Is your antibody a polyclonal rabbit or polyclonal goat or mouse monoclonal or rat
monoclonal?
3, What is the tissue you are testing, rabbit tissue or rat tissue or mouse tissue?

  

Source: Abagyan, Ruben - School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego

 

Collections: Biology and Medicine