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Am. J. Trop. Med. Hvg.. 54(5). 1996. pp. 475"480 Copyright C 1996by The American Societyof Tropical Medicine and Hygiene
 

Summary: Am. J. Trop. Med. Hvg.. 54(5). 1996. pp. 475"480
Copyright C 1996by The American Societyof Tropical Medicine and Hygiene
ISOLATION AND CHARACTERIZATION OF THE TSETSE THROMBIN
INHIBITOR: A POTENT ANTITHROMBOTIC PEPTIDE FROM THE
SALIVA OF GLOSSINA MORSITANS MORSITANS
MICHAEL CAPPELLO, PETER W. BERGUM, GEORGE P. VLASUK,
BARBARA A. FURMIDGE, DAVID I. PRITCHARD, @DSERAP AKSOY
Departments of Pediatrics and Internal Medicine, Yale University School of Medicine, New Haven,
Connecticut; Corvas International, Inc., San Diego, California; Department of Life Science,
University of Nottingham, Nottingham, United Kingdom
Abstract. A potent and specific inhibitor of the human coagulation protease thrombin was identified in salivary
gland extracts of the tsetse fly, Glossina morsitans morsitans, an important vector of African Irypanosomiasis. This
low molecular weight peptide (MW = 3,530 Da as determined by laser desorption mass spectrometry) was purified
using a combination of size-exclusion chromatography and reverse-phase, high-performance liquid chromatography,
respectively. Amino terminal sequencing of the purified protein reveals no homology to any previously identified
serine protease inhibitor or naturally occurring anticoagulant. The tsetse thrombin inhibitor (TFI) is a stoichiometric
inhibitor of thrombin, with an apparent equilibrium dissociation inhibitory constant (@@)I') of 584 X l0'@ M. In
addition, it is also a potent inhibitor of thrombin-induced platelet aggregation. Like other hematophagous arthropods,
tsetse flies appear to have evolved a novel protease inhibitor capable of antagonizing host hemostasis and facilitating
blood feeding.

  

Source: Aksoy, Serap - School of Public Health, Yale University

 

Collections: Biology and Medicine