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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 243, 664668 (1998) ARTICLE NO. RC988151
 

Summary: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 243, 664­668 (1998)
ARTICLE NO. RC988151
Two Subunits of the FoF1-ATPase Are Phosphorylated
in the Inner Mitochondrial Membrane
Andre´ Struglics,* Kenneth M. Fredlund,,1
Ian M. Møller, and John F. Allen*,2
*Plant Cell Biology, Lund University, Box 7007, S-220 07 Lund, Sweden; and
Plant Physiology, Lund University, Box 117, S-221 00 Lund, Sweden
Received January 15, 1998
(6­8), but the plant mitochondrial phosphoproteins are
Inside-out submitochondrial particles from potato unidentified and their regulatory role is not known.
tuber mitochondria were incubated with [g-32
P]ATP. Previous work on protein phosphorylation in plant mi-
More than 16 phosphorylated polypeptides were de-
tochondria has been carried out on intact mitochondria
tected by autoradiography on an SDS-gel. Two phos-
prepared from potato tubers where inner membrane
phoproteins, migrating at 22 and 28 kDa, were excised
phosphoproteins are not easily distinguished from
from the SDS-gel, electroeluted, and purified further

  

Source: Allen, John F. - School of Biological and Chemical Sciences, Queen Mary, University of London

 

Collections: Renewable Energy; Biology and Medicine