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Insect Biochemistry and Molecular Biology 32 (2002) 16631671 www.elsevier.com/locate/ibmb

Summary: Insect Biochemistry and Molecular Biology 32 (2002) 16631671
Proventriculus-specific cDNAs characterized from the tsetse,
Glossina morsitans morsitans
Zhengrong Hao, Serap Aksoy
Department of Epidemiology and Public Health, Section of Vector Biology, Yale University School of Medicine, 60 College St, 606 LEPH, New
Haven, Connecticut 06510, USA
Received 14 February 2002; received in revised form 9 May 2002; accepted 25 May 2002
Peritrophic matrix (peritrophic membrane or PM) is an important structure in the gut of most insects at some stage in their
development. It is composed of chitin, proteins and proteoglycans. Multiple roles for the PM ranging from partitioning of digestive
enzymes and food to protection of gut epithelial cells from viral and parasitic invasion have been proposed. While most adult
members of Diptera have a Type I PM synthesized in response to a blood meal, the medically and agriculturally important vector
insect, tsetse has a sleeve-like Type II PM which is constitutively synthesized by cells in the proventriculus (cardia). Using a
differential hybridization approach, we have identified three abundant cDNAs from a proventriculus cDNA library of Glossina
morsitans morsitans: GmPro1, GmPro2 and GmPro3. DNA sequence analysis indicates that GmPro1 and GmPro2 share similarities
with the peritrophin-15 family of larval PM proteins, while GmPro3 is a member of the serine protease family. Northern analysis
indicates that transcripts for all three cDNAs are preferentially expressed in the proventriculus tissue. The expression profile of
these genes in response to the presence of trypanosome indicates that transcription of GmPro1 is increased in the presence of
parasites (immune sensitive), while the other two are not affected. Western analysis using antibodies developed against the recombi-


Source: Aksoy, Serap - School of Public Health, Yale University


Collections: Biology and Medicine