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Proc. Natl. Acad. Sci. USA Vol. 94, pp. 1246712472, November 1997
 

Summary: Proc. Natl. Acad. Sci. USA
Vol. 94, pp. 12467­12472, November 1997
Genetics
Intron self-complementarity enforces exon inclusion in a
yeast pre-mRNA
KENNETH JAMES HOWE AND MANUEL ARES, JR.*
Center for the Molecular Biology of RNA, Biology Department, University of California, Santa Cruz, CA 95064
Edited by John Abelson, California Institute of Technology, Pasadena, CA, and approved September 15, 1997 (received for review July 18, 1997)
ABSTRACT Skipping of internal exons during removal of
introns from pre-mRNA must be avoided for proper expres-
sion of most eukaryotic genes. Despite significant understand-
ing of the mechanics of intron removal, mechanisms that
ensure inclusion of internal exons in multi-intron pre-mRNAs
remain mysterious. Using a natural two-intron yeast gene, we
have identified distinct RNA­RNA complementarities within
each intron that prevent exon skipping and ensure inclusion
of internal exons. We show that these complementarities are
positioned to act as intron identity elements, bringing to-
gether only the appropriate 5 splice sites and branchpoints.
Destroying either intron self-complementarity allows exon

  

Source: Ares Jr., Manny - Department of Molecular, Cell, and Developmental Biology, University of California at Santa Cruz

 

Collections: Biology and Medicine