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Cell, Vol. 29, 551-559, June 1982, Copyright 0 1982 by MIT Directed Transposon Tn5 Mutagenesis and
 

Summary: Cell, Vol. 29, 551-559, June 1982, Copyright 0 1982 by MIT
Directed Transposon Tn5 Mutagenesis and
Complementation Analysis of Rhizobium meliloti
Symbiotic Nitrogen Fixation Genes
Gary B. Ruvkun, Venkatesan Sundaresan and
Frederick M. Ausubel
Department of Cellular and Developmental Biology
Harvard University
Cambridge, Massachusetts 02138
Summary
An 18 kb region adjacent to and surrounding the
genes for nitrogenase (nif) was cloned from the
genome of the symbiotic nitrogen-fixing species
Rhizobium meliloti. A total of 31 Tn5 insertions in
the nif region were constructed and assayed for
their effect on symbiotic nitrogen fixation (Fix phe-
notype). Fix- insertions were found in two clusters,
one 6.3 kb and a second at least 5.0 kb, separated
by a 1.6 kb region not containing essential sym-
biotic genes. The locations of at least three tran-

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine