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A Method for Generating Transgenic Frog Embryos Shoko Ishibashi, Kristin L. Kroll and Enrique Amaya
 

Summary: A Method for Generating Transgenic Frog Embryos
Shoko Ishibashi, Kristin L. Kroll and Enrique Amaya
1. Introduction
1.1. Summary
The amphibian embryo has classically been one of the best systems for
elucidating the molecular mechanisms of early development, in particular for
studies of mesodermal and neural induction. Amphibian embryos develop
externally and are large and robust. Therefore tissues can be dissected, isolated
or transplanted with high precision and ease in these embryos. In addition, it is
relatively easy to manipulate the expression of gene products by injecting in vitro
transcribed RNAs into developing embryos. However, since RNAs are translated
soon after injection, this method has been used mainly for studying early stages
of development. Manipulating genes specifically during later stages of
development requires fine control over the time and place of expression, which
can only be achieved through transgenic technology. In this chapter we describe
a very efficient method of transgenesis developed for Xenopus laevis and
Xenopus tropicalis.
1.2. Background
Understanding the molecular basis of pattern formation and differentiation in
frog embryos was previously hindered by the lack of a system for temporal and

  

Source: Amaya, Enrique - Healing Foundation Centre & Developmental Biology, Faculty of Life Sciences, University of Manchester

 

Collections: Biology and Medicine