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Journal of Neuroscience Methods 113 (2002) 199206 A new method for the investigation of capillary structure
 

Summary: Journal of Neuroscience Methods 113 (2002) 199­206
A new method for the investigation of capillary structure
D.A. Tata a
, B.J. Anderson a,b,
*
a
Department of Psychology, State Uni6ersity of New York at Stony Brook, Stony Brook, New York, NY 11794-2500, USA
b
Program in Neurobiology and Beha6ior, State Uni6ersity of New York at Stony Brook, Stony Brook, New York, NY 11794-2500, USA
Received 19 July 2001; received in revised form 10 October 2001; accepted 10 October 2001
Abstract
Numerous physiological conditions as well as behavioral conditions have been shown to influence central nervous system
vascular structure. Many of the methods used to investigate these structural alterations take advantage of the visibility of viscous
substances (e.g. India ink in gelatin) perfused into the vasculature. The high viscosity of the solution, however, can cause
incomplete vessel perfusion. The aim of the present study was to test whether or not capillaries seen in tissue perfused with
fixative, embedded in celloidin and stained with Methylene Blue­Azure II (n=6) could be a useful alternative for the
investigation of brain vascular structure. The method was compared to tissue from six rats perfused with India ink in gelatin and
stained with cresyl violet. Qualitatively, vessels in the standard perfused tissue embedded in celloidin yielded clear vessels with
stained pericytes. The two methods did not differ in branch point to cell ratio, length of individual capillaries, vessel length per
mm3

  

Source: Anderson, Brenda - Department of Psychology, SUNY at Stony Brook

 

Collections: Biology and Medicine