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Summary: Spectral restoration from low signal-to-noise, distorted NMR
signals: application to hyphenated capillary electrophoresis-NMR
Yu Li,a,b
Michael E. Lacey,b,c
Jonathan V. Sweedler,b,c
and Andrew G. Webba,b,d,*
a
Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
b
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
c
School of Chemical Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
d
Physikalisches Institut, Universitaet Wuerzburg, Germany
Received 22 July 2002; revised 11 February 2003
Abstract
In capillary electrophoresis separations coupled to NMR signal detection using small solenoidal coils, electrophoretic currents
cause substantial distortion in the NMR spectral linewidths and peak heights, distortions which cannot be fully counteracted
through shimming. The NMR spectra also have a low signal-to-noise ratio due to the small amounts of material, typically <1 nmol,
associated with such microseparations. This study proposes a two-step, signal processing method to restore spectral lines from the
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