Home

About

Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network
FAQHELPSITE MAPCONTACT US


  Advanced Search  

 
Plant Science 166 (2004) 6979 Trapping and characterization of cold-responsive
 

Summary: Plant Science 166 (2004) 6979
Trapping and characterization of cold-responsive
genes from T-DNA tagging lines in rice
Sang-Choon Leea, Ji-Youn Kima, Sung-Hyun Kima, Soo-Jin Kima, Kang Leea, Soon-Ki Hana,
Hong-Seok Choia, Dong-Hoon Jeongb, Gynheung Anb,1, Seong-Ryong Kima,
a Department of Life Science, Sogang University, Seoul 121-742, South Korea
b Department of Life Science, Pohang University of Science and Technology, Pohang 790-784, South Korea
Received 19 August 2003; received in revised form 19 August 2003; accepted 20 August 2003
Abstract
To identify low-temperature-responsive genes in rice, we screened gus-trapped T-DNA tagging lines that had been subjected to cold
stress at 5
C. Out of 15,586 lines, 81 (0.52%) showed cold-responsive GUS activity. Among the 62 lines we selected for further study, 53
showed increased GUS activity while 9 showed less. In addition, 16 of those 62 lines were influenced by abscisic acid (ABA), suggesting an
ABA-dependent cold response. We used inverse PCR or thermal asymmetric interlaced (TAIL) PCR to identify 37 tagged genes from those
lines, and further characterized two of them at the molecular level. The OsRLK1 gene, which encodes a putative leucine rich repeat (LRR)-type
receptor-like protein kinase, was inducible by cold and salt stresses. The OsDMKT1 gene, encoding a putative demethylmenaquinone methyl-
transferase (DMKT), was also inducible by low temperatures, but not by high salt or drought. The T-DNA-tagged OsDMKT1 gene also
co-segregated with the cold-inducible gus gene. Our results indicate that the T-DNA tagging lines are useful in obtaining the stress-responsive
genes in rice.
2003 Elsevier Ireland Ltd. All rights reserved.

  

Source: An, Gynheung - Department of Life Science, Pohang University of Science and Technology

 

Collections: Biotechnology; Biology and Medicine