Home

About

Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network
FAQHELPSITE MAPCONTACT US


  Advanced Search  

 
Transcytosis of Polymeric Immunoglobulin A in Polarized MadinDarby Canine Kidney Cells
 

Summary: 12
Transcytosis of Polymeric Immunoglobulin A in
Polarized Madin­Darby Canine Kidney Cells
Asli Oztan, Christine Rondanino, and Gerard Apodaca
1 Introduction........................................................................................................................ 157
2 Materials ............................................................................................................................ 159
3 Methods.............................................................................................................................. 162
4 Notes .................................................................................................................................. 168
References................................................................................................................................ 170
Summary The transcytotic pathway allows for the bidirectional transport of
endocytosed solutes, lipids, and proteins between the two membrane domains of
polarized epithelial cells while maintaining the functional integrity of the epithe-
lial tissue. A method is described to measure basolateral-to-apical transcytosis of
immunoglobulin A (IgA) in polarized Madin­Darby canine kidney (MDCK) cells
expressing the polymeric immunoglobulin receptor (pIgR). The cells are grown on
porous Transwell filter supports, and radiolabeled 125
I-immunoglobulin A (IgA) is
internalized from the basolateral pole of MDCK cells. During a subsequent 2-h
chase, the amount of 125
I-IgA that is recycled, degraded, or transcytosed is quanti-

  

Source: Apodaca, Gerard - Departments of Medicine & Cell Biology and Physiology, University of Pittsburgh

 

Collections: Biology and Medicine