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IL-4 is required to prevent filarial nematode development in resistant but not susceptible strains of mice

Summary: IL-4 is required to prevent filarial nematode development in resistant but
not susceptible strains of mice
Laetitia Le Goff, Tracey J. Lamb, Andrea L. Graham, Yvonne Harcus, Judith E. Allen*
Institute of Cell, Animal and Population Biology, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh EH9 3JT, UK
Received 8 May 2002; received in revised form 11 June 2002; accepted 11 June 2002
The murine Litomosoides sigmodontis model of filarial infection provides the opportunity to elucidate the immunological mechanisms that
determine whether these nematode parasites can establish a successful infection or are rejected by the mammalian host. BALB/c mice are
fully susceptible to L. sigmodontis infection and can develop patent infection, with the microfilarial stage circulating in the bloodstream. In
contrast, mice on the C57BL background are largely resistant to the infection and never produce a patent infection. In this study, we used IL-4
deficient mice on the C57BL/6 background to address the role of IL-4 in the development of L. sigmodontis parasites in a resistant host. Two
months after infection, adult worm recovery and the percentage of microfilaraemic mice in infected IL-4 deficient mice were comparable
with those of the susceptible BALB/c mice while, as expected, healthy adults were not recovered from wild type C57BL/6 mice. The
cytokine and antibody responses reveal that despite similar parasitology the two susceptible strains (BALB/c and IL-4 deficient C57BL/6)
have markedly different immune responses: wild type BALB/c mice exhibit a strong Th2 immune response and the IL-4 deficient C57BL/6
mice exhibit a Th1 response. We also excluded a role for antibodies in resistance through infection of B-cell deficient C57BL/6 mice. Our
data suggest that the mechanisms that determine parasite clearance in a resistant/non-permissive host are Th2 dependent but that in a
susceptible/permissive host, the parasite can develop in the face of a Th2 dominated response. q 2002 Australian Society for Parasitology
Inc. Published by Elsevier Science Ltd. All rights reserved.
Keywords: Litomosoides; Th1/Th2; Murine filariasis


Source: Allen, Judith - School of Biological Sciences, University of Edinburgh
Read, Andrew - Center for Infectious Disease Dynamics & Department of Entomology, Pennsylvania State University


Collections: Biology and Medicine; Environmental Sciences and Ecology