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Plant Molecular Biology 52: 761773, 2003. 2003 Kluwer Academic Publishers. Printed in the Netherlands.
 

Summary: Plant Molecular Biology 52: 761773, 2003.
2003 Kluwer Academic Publishers. Printed in the Netherlands.
761
Transgene structures in T-DNA-inserted rice plants
Sung-Ryul Kim1,2, Jinwon Lee1, Sung-Hoon Jun1, Sunhee Park1, Hong-Gyu Kang1, Soontae
Kwon2 and Gynheung An1,
1National Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang
University of Science and Technology (POSTECH), Pohang 790-784, Republic of Korea (author for correspon-
dence; email genean@postech.ac.kr); 2Department of Horticulture and Breeding, Andong National University,
Andong 760-749, Republic of Korea
Received 19 December 2002; accepted in revised form 5 March 2003
Key words: border, flanking sequence, Oryza sativa, repeat, rice, T-DNA integration, vector backbone
Abstract
T-DNA is commonly used for delivery of foreign genes and as an insertional mutagen. Although ample information
exists regarding T-DNA organization in dicotyledonous plants, little is known about the monocot rice. Here, we
investigated the structure of T-DNA in a large number of transgenic rice plants. Analysis of the T-DNA borders
revealed that more than half of the right ends were at the cleavage site, whereas the left ends were not conserved
and were deleted up to 180 bp from the left border (LB) cleavage site. Three types of junctions were found between
T-DNA and genomic DNA. In the first, up to seven nucleotide overlaps were present. The frequency of this type
was much higher in the LB region than at the right border (RB). In the second type, which was more frequent in

  

Source: An, Gynheung - Department of Life Science, Pohang University of Science and Technology

 

Collections: Biotechnology; Biology and Medicine