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Both Microtubules and Actin Filaments Are Required for Efficient Postendocytotic Traffic of the Polymeric Immunoglobulin Receptor
 

Summary: Both Microtubules and Actin Filaments Are Required for Efficient
Postendocytotic Traffic of the Polymeric Immunoglobulin Receptor
in Polarized Madin-Darby Canine Kidney Cells*
(Received for publication, August 16, 1996, and in revised form, December 18, 1996)
Christopher J. Maples, Wily G. Ruiz, and Gerard Apodaca
From the Laboratory of Epithelial Cell Biology, Renal/Electrolyte Division of the Department of Medicine,
and Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, Pennsylvania 15213
It has been postulated that membrane traffic in polar-
ized epithelial cells requires both actin filaments and
microtubules. We have tested this hypothesis by analyz-
ing the effect of cytochalasin D (cytoD; an actin-disrupt-
ing agent), by itself or in combination with nocodazole
(a microtubule depolymerizing agent), on postendocytic
traffic in Madin-Darby canine kidney cells. CytoD treat-
ment inhibited basolateral to apical transcytosis of IgA
in polymeric immunoglobulin receptor-expressing cells
by approximately 45%, but had little effect on basolat-
eral recycling of transferrin. Apical recycling of IgA was
also inhibited by approximately 20%. Like nocodazole,
cytoD acted at an early step in transcytosis, and inhib-

  

Source: Apodaca, Gerard - Departments of Medicine & Cell Biology and Physiology, University of Pittsburgh

 

Collections: Biology and Medicine