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Molecular Cell Short Article

Summary: Molecular Cell
Short Article
Dynamics and Variability of ERK2 Response
to EGF in Individual Living Cells
Cellina Cohen-Saidon,1,3 Ariel A. Cohen,1,3 Alex Sigal,1,2 Yuvalal Liron,1 and Uri Alon1,*
1Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100 Israel
2Present address: Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
3These authors contributed equally to this work
*Correspondence: uri.alon@weizmann.ac.il
DOI 10.1016/j.molcel.2009.11.025
Signal-transduction cascades are usually studied on
cell averages, masking variability between individual
cells. To address this, we studied in individual cells
the dynamic response of ERK2, a well-characterized
MAPK signaling protein, which enters the nucleus
upon stimulation. Using fluorescent tagging at the
endogenous chromosomal locus, we found that
cells show wide basal variation in ERK2 nuclear
levels. Upon EGF stimulation, cells show (1) a fold-


Source: Alon, Uri - Departments of Molecular Cell Biology & Physics of Complex Systems, Weizmann Institute of Science


Collections: Biology and Medicine