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Molecular Ecology Resources (2008) 8, 434436 doi: 10.1111/j.1471-8286.2007.01983.x 2007 The Authors
 

Summary: Molecular Ecology Resources (2008) 8, 434­436 doi: 10.1111/j.1471-8286.2007.01983.x
© 2007 The Authors
Journal compilation © 2007 Blackwell Publishing Ltd
Blackwell Publishing Ltd
PERMANENT GENETIC RESOURCES
Isolation and characterization of polymorphic microsatellite
loci in common evening primrose (Oenothera biennis)
E. L. LARSON,* S. M. BOGDANOWICZ,* A. A. AGRAWAL,* M. T. J. JOHNSON and R. G. HARRISON*
*Department of Ecology and Evolutionary Biology, Corson Hall, Cornell University, Ithaca, NY 14853, USA, Department of Biology,
Duke University, Durham, NC 27708, USA
Abstract
We developed nine polymorphic microsatellite loci for evening primrose (Oenothera biennis).
These loci have two to 18 alleles per locus and observed heterozygosities ranging from
0 to 0.879 in a sample of 34 individuals. In a pattern consistent with the functionally
asexual reproductive system of this species, 17/36 pairs of loci revealed significant linkage
disequilibrium and three loci showed significant deviations from Hardy­Weinberg equi-
librium. The loci will be informative in identifying genotypes in multigenerational field
studies to assess changes in genotype frequencies.
Keywords: heterozygosity, microsatellite, Oenothera biennis, Onagraceae, permanent translocation
heterozygote

  

Source: Agrawal, Anurag - Department of Ecology and Evolutionary Biology & Entomollogy, Cornell University
Johnson, Marc - Department of Plant Biology, North Carolina State University

 

Collections: Biology and Medicine; Environmental Sciences and Ecology