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Multi-color DNA Analysis in an Optofluidic Chip C. Dongre, H.J.W.M. Hoekstra, and M. Pollnau

Summary: Multi-color DNA Analysis in an Optofluidic Chip
C. Dongre, H.J.W.M. Hoekstra, and M. Pollnau
Sorting and sizing of DNA molecules by capillary electrophoresis (CE) within the human genome
project have enabled the genetic mapping of various illnesses. By the use of miniaturized lab-on-a-
chip devices, on-chip-integrated DNA sequencing and genetic diagnostics have become feasible. The
inherent advantages of microfluidic CE separation of differently sized DNA molecules, high-speed
operation and low reagent volumes, in combination with laser-induced fluorescence detection, result
in optofluidic integration toward on-chip bio-analysis tools which aim at solving real-life challenges
in medicine, e.g. identification of genomic deletions or insertions associated with genetic illnesses.
1. Optofluidic integration in an electrophoretic microchip
The lay-out of our optofluidic chip is presented in Fig. 1. Chips were fabricated in a two-step
procedure. Firstly, the microfluidic channel network and microfluidic reservoirs were patterned
photolithographically and wet-etched in fused silica glass and then sealed off by bonding another
piece of fused silica glass on top (LioniX BV). The chip has dimensions of 55 mm 5.5 mm 1 mm
and the microfluidic channels have a cross section of ~110 m width and ~50 m depth.
In a second step, the optical waveguide was inscribed into the bulk of such a fused silica chip by
fs-laser writing using a Ti:Sapphire laser [1]. Employing astigmatic beam shaping, an elliptical cross
section of the written waveguide was obtained, with a major diameter of ~50 m in the vertical
direction, in order to excite the maximum possible volume of the microfluidic channel, while the
minor diameter in the horizontal direction is ~12 m in order to retain a high spatial resolution along


Source: Al Hanbali, Ahmad - Department of Applied Mathematics, Universiteit Twente
Twente, Universiteit - Department of Science and Technology, Optical Techniques Group


Collections: Engineering