| | |
Summary: Fourier analysis and systems identification of the p53
feedback loop
Naama Geva-Zatorskya,1
, Erez Dekela,1
, Eric Batchelorb
, Galit Lahavb
, and Uri Alona,2
a
Department of Molecular Cell Biology and Department of Physics of Complex Systems, Weizmann Institute of Science, Rehovot 76100, Israel; and
b
Department of Systems Biology, Harvard Medical School, Boston, MA 02115
Edited by John J. Tyson, Virginia Polytechnic Institute and State University, Blacksburg, VA, and accepted by the Editorial Board June 14, 2010 (received for
review January 31, 2010)
A key circuit in the response of cells to damage is the p53mdm2
feedback loop. This circuit shows sustained, noisy oscillations in
individual human cells following DNA breaks. Here, we apply an
engineering approach known as systems identification to quantify
the in vivo interactions in the circuit on the basis of accurate meas-
urements of its power spectrum. We obtained oscillation time
courses of p53 and Mdm2 protein levels from several hundred cells
|
