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Directed Evolution DOI: 10.1002/anie.200702616
 

Summary: Directed Evolution
DOI: 10.1002/anie.200702616
Engineered Alkane-Hydroxylating Cytochrome P450BM3 Exhibiting
Nativelike Catalytic Properties**
Rudi Fasan, Mike M. Chen, Nathan C. Crook, and Frances H. Arnold*
Cytochrome P450 enzymes (P450s) are exceptional oxygen-
ating catalysts[1]
with enormous potential in drug discovery,
chemical synthesis, bioremediation, and biotechnology.[2,3]
Compared to their natural counterparts, however, engineered
P450s often exhibit poor catalytic and cofactor coupling
efficiencies.[3]
Obtaining native-like catalytic proficiencies is a
mandatory first step towards utilizing the power of these
versatile oxygenases in chemical synthesis.
Cytochrome P450BM3 (119 kDa, B. megaterium) catalyzes
the subterminal hydroxylation of long-chain (C12C20) fatty
acids.[4]
Its high activity and catalytic self-sufficiency (heme
and diflavin reductase domains are fused in a single polypep-

  

Source: Arnold, Frances H. - Division of Chemistry and Chemical Engineering, California Institute of Technology

 

Collections: Chemistry; Biology and Medicine