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Biomaterials 31 (2010) 900-907 Contents lists available at ScienceDirect

Summary: Biomaterials 31 (2010) 900-907
Contents lists available at ScienceDirect
journal homepage: www.elsevier.com/locate/biomaterials
The role of antibody synergy and membrane fluidity in the vascular
targeting of immunoliposomes
Rico C. Cunawan, Debra T. Auguste*
School of Engineeing and Applied Sciences, Haruard university, 29 Oxlord St., Cambidge, MA 02138, USA
Targeted drug delivery to inflamed or injured vascular endothelial cells (ECs) and smooth muscle cells
(SMCs) may provide a precise and effective therapeutic treatment for cardiovascular diseases. Upregu-
lation of cytokine-regulated cell surface receptors, intercellular cell adhesion molecule-l (ICAM) and
endothelial-leukocyte adhesion molecule-1 (ELAM), on ECs and SMCs are used to target drug delivery
vehicles. Recent studies demonstrate clustering of these molecules in lipid rafts may affect binding due to
a nonhomogenous presentation of antibodies. We hypothesized that altering the antibody ratio for ICAM
and ELAM (alCAM:aELAM) and mobility would influence cellular targeting. To alter antibody mobility,
liposomes were prepared from either 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOpC, C18.1, Tm-
-20'C) or 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DppC, Crc.s, T6:42 "C) which are in the
liquid crystalline (Lo) and gel phase (Lg) at 37'C, respectively. We report that cellular binding ofDOpC
immunoliposomes by ECs is maximal at an equimolar ratio of aICAM:aELAM whereas DPPC immunoli-


Source: Auguste, Debra T. - School of Engineering and Applied Sciences, Harvard University


Collections: Materials Science; Biology and Medicine