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Gene 252 (2000) 8393 www.elsevier.com/locate/gene

Summary: Gene 252 (2000) 8393
A family of genes with growth factor and adenosine deaminase
similarity are preferentially expressed in the salivary
glands of Glossina m. morsitans
Song Li, Serap Aksoy *
Yale University School of Medicine, Department of Epidemiology and Public Health, New Haven, CT 06510, USA
Received 5 May 1999; received in revised form 25 April 2000; accepted 26 April 2000
Received by J.A. Engler
A cDNA library constructed from salivary glands of tsetse fly, Glossina morsitans morsitans (Diptera: Glossinidae), was
differentially screened, and two related full-length cDNAs were molecularly characterized: tsetse salivary growth factor, TSGF-1
and TSGF-2. The cDNAs encode for open reading frames (ORFs) of 494 and 506 aa, respectively, and display an overall 45%
amino acid identity and 61% similarity to one another. Both genes are preferentially expressed in the salivary glands of male and
female adult flies. In addition to salivary glands, both transcripts can be detected from the gut tissue. Only transcripts specific for
TSGF-2 are detected in ovary and testes tissues of adults as well as in puparia, while neither gene is expressed during the larval
developmental stages. The N-terminal region of both putative proteins contains a hydrophobic sequence with secretory signal
peptide characteristics, and analysis of proteins in saliva by Western blot indicates that both are secreted. Western blot analysis
indicates that TSGF-1 is synthesized at significantly higher levels than TSGF-2. The deduced protein sequences of both cDNAs
display extensive similarities to two other proteins: insect derived growth factor (IDGF) characterized from Sarcophaga peregrina


Source: Aksoy, Serap - School of Public Health, Yale University


Collections: Biology and Medicine